Genetic Regulation of Apoptosis and Organ Development

transmittable economy of programmed prison cubicle termination and Organ in systema skeletaleationThis report reviews or so the ingre go alongnttic stoogeon of programmed cadre last and electronic organ phylogeny. The limited agents concur been primed(p) which cause these functions inner(a) an organism proves to us around racy sequential sy antecedents bear uponing carrell specialism which in wrick conks to the proliferation of the species. This report mainly gives you a clear bring out explanation about how carrel demolition and organ maturement f atomic number 18 to bum aroundher in a progressive manner in concern with the using of an organism. The comp championnts determined have been attested to be very helpful in the field of treatment of diseases prison term and time again. And the review in addition dissertates on how the model nematode Caenorhabditis elegans has been used efficiently to determine the suspensionrictive factors of develop ment and programmed kiosk terminal. Which sequenti anyy leads us to some predetermined definite advantages of the practicable determinations in the field of medicine.thither be an enormous number of carrells in the compassionate body and in altogether organisms. Including an exception for numerous some other bacteria and other microorganisms lower depressed the complication order. The basis of all(a) these cells argon ii butt ones namely mitosis and meiosis. To be more special in creation the fertilized egg is the source of all types of cells. From the fertilized egg in the humans to the innate litigate of mitosis and meiosis in the smallest of organisms all abide cell una selfsame(prenominal)iation. This attend in specify as bringing in the feature of speech of a peculiar(prenominal) cell to it specialised function. These cells fundamentally develop into motley types of cells from their sinkage gunpoint of interphase in mitosis. precisely to remember that the zygotic percentage point never determines the circumstantial function of the cell. Not only the newly make up cells brook eminence only when the mature cells or the adult stem cells to be more specific undergo variantiation to form specific wavers and then variety meat and aft(prenominal)ward to form a whole organism. The adult stem cells subsequently their process of differentiation transfer their characteristics to their daughter cells in such a way that the daughter cells in any case exhibit the very(prenominal) characteristics as their p bental generation. The cell differentiate along these lines that the whole sound structure of the cell exchangeable the cell size, cell structure, membrane potential and in time its response to signalises alter. On the contrary cell death a ilk plays a major part in cell differentiation and organ development. For congressman the process of metamorphosis in butterfly from larvae to the whole metamophosized butterf ly or from the tadpole to a frog. The deaths of many cells ar involved in this process, but very specific cells. programmed cell death is the word given for a programmed process of cell death without which the development of organs or any higher(prenominal)(prenominal) organism is nigh un deally to happen. All the processes mentioned above be the consequences of gene manipulation within the cell. These atomic number 18 controlled by specific genes within a cell giving out certain signals as and when needed for different processes. determine the genes that are involved in these processes is called as here(predicate)ditary standard. Genetic regulation or gene evaluation plays a vital position in the field of medicine.This article establishes about the use of Caenorhabditis elegans, a naive nematode worm as a specimen for determining the genetic regulation of organ development and programmed cell death or programmed cell death. This specific species was short listed among many others as it had a very short time spanned cell cycle (Wood and William, 1988). Which consisted of only 959 adult cells in its generation cells make it very easy to analyse and determine the genetic regulation (Brenner, 1974). On the whole three scientists moulded on determining the genetic regulation of programmed cell death and organ development.Sir John Sulston was the first one among the three to initiate the sample starting with developing all the techniques to study cell division in the nematode worm from its stage of a fertilized egg to a screwly mature adult stage of the worm. (Sulston and Horvitz, 1977).Dr. H. Robert Howitz continued the work of Sir John Sulston by putting forward the question whether in that location was a genetic enactment for all death and development processes taking push through in an organism. A specific genetic programmed that he suggested might be and determined the genetic regulations for the same processes in the worm (Jonathan and Ro bert, 1978).Dr.Sydney Brenner played his part by proving the work done by the previous scientists on determining the specific genes. He mutated those specific genes involved in the processes by using EMS or ethyl radical Methane Sulphonate. This landed up on the result that, when these genes are mutated the organ development does not purpose beat and consequentially lead to the death of the organism (Brenner, 1973) (Jonathan and Sydney Brenner, 1978).The work of all the three scientists helped in landing up in a theory and sampleal proof of genetic regulation of caspase-mediated cell death and organ development and as well that there is a major continuative betwixt both the processes for the survival of the organism. The use of nematode worm was considered because it is unmanageable to determine the same in higher wildcats. The genes like Ced-3 and Ced-4 were principally determined to be the genetic regulators of programmed cell death and the proteins which laws for the initiation of these genes were used for degrading the deoxyribonucleic acid after caspase-mediated cell death. Also making an understanding on how the dead cell is eliminated after the process of programmed cell death. It was proposed that the same regulations likewise appropriate place in higher organisms including humans as one of them with the help of homologous proteins like Apaf-1 in humans replacing CED-4 in C.elegans (Hua and William, 1997).The mannish and the hermaphrodite are differentiated by the morphology or by their internal organs. The male nematode is call backd to have 959 cells in its mature adult stage and makes it very easy to determine the genetic regulation. The picture above gives the askance view of dissected C.elegans. The lateral dissected view cl primeval shows the ease of the organism and also on why the organism was narrowed down to study upon. (Sulston and Horvitz, 1977)This report mainly concentrates on the determination of genes involved in th e process and how the two processes of development and death are linked in the complete life cycle of an organism. Determining the genetic regulation of the same plays a vital role in curing a authorize full of dreadful diseases like contributecer, AIDS and Myocardial Infarction (Thompson, 1995). The unbeatable growth of cells subjugateing the process of programmed cell death in the case of derrierecer (Morgan et al., 2006) and the death of cells oppressing the process of development and initiating the process of caspase-mediated cell death by activating all the available pamphlets (Explained in detailed in the proceeding pages) for the process of apoptosis in the case of AIDS (Perez et al., 2008). Determining the genes involved or genetic regulation has a major role in the treatment of these diseases. As genes and gene cryptology are the bases of every full of life organism in this universe.Genetic convention of caspase-mediated cell death and Organ DevelopmentTo just basically relieve about genetic regulation sooner getting slurred into the degree of the paper, genetic regulation is the process of turning on or turning of the genes that are needed and those of which are not needed respectively. The first ever gene regulation developments were on the lac and the trp operon model. Basically it is a organisation used for saving up the enzymes and using them whenever necessary and not expend them by accumulating them by continuously producing them on the contrary. These are helped by the genes. And for bump understanding s schematic is given below of the overall process of genetic regulation.Introduction to apoptosisApoptosis is derived from the Greek language kernel dropping off or falling off of parts. This I suppose does not give the appropriate meaning but the term was coined jibe to the preliminary discoveries of researchers regarding the same. The term was titled to fit the process as there were findings and literature that stated th e dropping of all organelles (not literally) of the cell after the depletion of the cell wall in the continuum of processes of apoptosis. The term generally means programmed cell death, which is defined as the well timed suicide of the cells by gene regulation as and when needed by the organism. This is the exact process that takes place in all organisms from a single celled to a multicellular complex organism. Apoptosis make up ones minds certain morphological features of the cell confidential information to its death in the coming cycles of apoptosis. The morphological changes include bleebing, loss of cell membrane, asymmetry of the cell, fragmentation of the desoxyribonucleic acid and many other structural and functional changes (Alberts et al., 2008).Atrophy is caused in the nett stages of apoptosis which mess lead to the complete destruction of the cells. The process was primarily considered to be incomplete as it was not known how the cells were dissolved after their de ath as the organelles after the cell death would cause to create an unwanted mass in the organism. just latter it was determined that the cells after the process of apoptosis ended up in creating apoptotic bo poop outs which were engulfed by other cells using pseudo arms and then were dissolved using the proteins that code for the gene to regulate the process of apoptosis (Walker and Sikorska 1994). During the years of the primary experimentation this process was written along with the process of necrosis which was the premature death of cells due to external effects like toxins, hazardous chemicals or radiations. But further experimentation and trials proved that apoptosis was a self-inducing component of cells for their suicide in order that the follow up oning processes of organ development takes place without obstructions. When human trials are concerned it is to be noted that about 50 to 70 billion cells die each day in the same process, where at the same time daughter cells are generated.Cell death plays an key role in organ development and weave homeostasis. The regulation of cell proliferation by Programmed Cell expiration (Apoptosis) contributes to organ and tissue development and differentiation to a great extent. Depending upon the time and clinical tincts many genes change their rule during organ development. The success of organ development completely depends upon the inter effect between the maintenance of cell survival and cell death. Cell death plays a significant role in promoting growth and tissue development of an organism. largely when cell death occurs, the quest points are to be taken into account,Development of regular tissues and cell death.Regulation of cell cycle and expression of genes.Determining the cell death parcel.The alley acts as a molecular invest for therapy (Prof. Dr. M. Nurhalim Shahib, 2001).When cells die, the contents of the cell are released in the surrounding and it causes inflammation or swelling which is termed as necrosis. When cells die during ordinary development or tissue homeostasis, they tend to condense and shrink and the dead cells are phagocytised by neighbouring cells before the contents of the cell get leaked in the surrounding. They do not nominate any inflammatory response unlike the necrosis. This process is termed as Apoptosis or Programmed Cell Death (Kerr et al., 1972).Apoptotic passagewaysExtracellular and intracellular pathways are the two terms that are concerned when there is a death of a cell taking place or a death of base of cells. Extracellular pathways are the subjecting of cells to toxin or hormones or growth factors which can in turn lead to the death of the cell. This not of much concern in our review as we are concentrating on the pathways of apoptosis. To get a draft knowledge about the death of the cells we should know that the death of the cells can take place by sensoring the signals from inside the cell or from the outside of the cell. Apop tosis mainly takes place by transducing signals from the inside of the cell. This process can be put into two main categories namely positive initiation and ostracise induction. Induction means the inhibition of the reaction and hence positive induction refers to the undergoing apoptosis reaction.To give a brief about the extracellular and intracellular processes. The intracellular processes will be explained in detail below and the extracellular process is simply the transducing of signals in case of unstable environment or the sequential crossing of the plasma membrane.Intracellular signalling has many different pathways which follow a definite sequence of steps. These including some like the binding of receptors by glucorticoids in the presence of high calcium concentration. All processes in the cells are initiated by enzymes specific enzymes that code for specific genes to be unrestrained for the following reaction to take place. Hence in the contrary these proteins or enzyme s can also be used to inhibit the reaction by finding out the genes which code for the enzyme to be produced. The process of apoptosis is mainly targeted to the mitochondria of the cell which then ceases al well-nigh all the functions the cell.For explaining in detail the intracellular processes that are taking place we need some specific pathways for a plenteous understanding. Many pathways are proposed and on literature which we are going to discuss in brief in the following pages of my report.Mitochondrial Regulation of Apoptosis before long explaining about mitochondria, they are the power house of the cell and supplies energy for the complete survival of the cell without which the cell is not in existence. Obviously free to say that without the functionality of the mitochondria the cell is just another non living organism without any appropriate use. This vivid function of the bacteria is used by the apoptotic pathways. in that location are two pathways by which the cells ar e forced to death by inhibiting the function of the mitochondria. They are the intrinsic and extrinsic pathway. They both vary in the pathways but the end product is incessantly the death of the cell. (Susin et al., 1999).The intrinsic pathway is basically the swelling up of the mitochondria by the formation of pores. This is formed by the binding of Cytochrome C to the apoptotic protease activating factor and then followed by the process of apoptosis.On the other hand the extrinsic pathway is the simplification of the membrane potential of the mitochondrial membrane by the action of nitric oxide and also the increase of the perme superpower causing a leak of enzymes inside the mitochondria. This causes the intractable swelling of the cell and also causes blebbing. When the cells continue to swell it leads to the damage of the cellular membrane that crimsontually leads to cell death. These are reported to be caused by the SMACs which are stated to be secondary mitochondria deriv ed activation of caspases. This can be proved to be the right opposite to the intrinsic pathway as this process deactivates the pathway that inhibits apoptosis by binding to the inhibition of apoptosis proteins or namely IAP rather than the intrinsic pathway that directly induces apoptosis. (Mayer and Oberbauer, 2003). When we get to compare both the processes we tend to end up in a result which states that the damage of the cell is indistinguishable between the two.2.2.2 Caspase Independent PathwayCaspase in considered as the most burning(prenominal) protein in the beseeming function of the apoptosis cycle. But it is proved that the apoptosis can also take place without the activity of the caspase protein. This takes place by a binding factor known as Apoptosis Inducing factor in (Cande et al., 2002) which only unavoidably transduced signals rather than the caspase binding (Magali et al., 1999)2.2.3 signalize TransductionSignal transduction is generally described as the proce ss of transferring signals. The same as mentioned above this also involves both extracellular and intracellular signals. This process is also called as the shape shifting process as the morphology of the cell is completely changed after this process takes place. there are two different pathways tumor necrosis factor and Fas pathways namely.Figure 4 TNF and FAS Pathway within the cell. The draw gives an easier explanation for the Fas pathway including the caspase binding reaction. It also shows many other proteins involved in programmed cell death. The TNF and the Fas pathways are two completely different pathways with the same functionality as to programmed cell death. They wait on mainly in transferring signals not only from the inside the cells to the point of pathway commencement but also from the exterior of the cell to the important signal transducers inside the cell. (Philip, 2004).The TNF pathway or the Tumor Necrosis Factor pathway. The pathway is commenced by the binding of TNF R1 and TNF R2 which in turn leads to the initiation of caspase activating pathway which then eventually leads to the death of the cell. both(prenominal) the pathways to be mentioned are results of binding. This pathway was also proved to be leading to one too many activations of transcription factors, the cause of numerous diseases (Chen and Goeddel, 2002).The Fas pathway also follows the same binding pathway as the TNF pathway. unaccompanied that the activation of transcription factors is unlikely to happen and the binding here takes place in caspase 8 and caspase 10. Binding of Fas and Fas L takes place along with the Death Inducing Signaling Factors or DISCs (Wajant H, 2002).2.3 Extracellular Control of ApoptosisProgrammed cell death or apoptosis have found to be activated or stifleed by extracellular signals from other cells apart from the apoptotic cells by controlling the mechanism. These extracellular signals are sent majorly to prevent programmed cell death. (Raff , 1992). There are a few examples to demonstrate how the signals activate or suppress apoptosis.Most of the vertebral cells tend to undergo apoptosis when cultured at a low density with the secretion of extracellular signals on their own. For Example- Blastomeres have the ability to survive and divide even in the absence of extracellular signals. The cells from tissues that are made of only single cell types have the ability to produce self-survival signals (Biggers et al., 1971).In some cells, a combination of several signals from different cells is required for their survival in a long term aspect. Example- The vertebral neurons during development compete for signals for their survival which are secreted by the target cells that they innervate. In this process only half of the neurons get enough signals to survive and the rest undergoes programmed cell death. Therefore, the normal death can be prevented by injecting exogenous Nerve Growth Factors (NGF) to the neurons. Similarly w hen the genes that code for NGFs are inactivated or by addition of anti-NGF antibodies, all the neurons undergo Apoptosis. commonly both inactivation and blastoff of NGFs are carried in a neuron to provide a relaxation between the number of neurons innervating the target cell and the unwanted neurons that target strange cells (Levi, 1987).Some cells are triggered by programmed cell death inducing signals which suppresses the action of the signals that are answerable for the survival of the cell. Example- In amphibians like tadpoles, a arrogant induction occurs at the metamorphosis stage where the cells in the tail undergo apoptosis due to the increase of thyroid hormone in the assembly line and this facilitates the resorption of the tail (Kerr et al., 1974).2.4 Overall Process of Apoptosis- Morphological ConcernThe contrive above gives us an easier understanding of the morphology of the cells in the course of Apoptosis. The diagram gives us a clear cut explanation about how normal cells receive signals and then followed by cell shoplifting and nuclear dud leading to death and formation of apoptotic bodies to the complete lysis of the cell. (Philip, 2004).ObservationCausesCell shrinkage and roundingBreakdown of proteinaceous cytoskeleton by caspase.Density of CytoplasmSignal transduction by TNF pathway or Mitochondrial regulationTight wadding of organellesSignal transduction by TNF pathway or Mitochondrial regulation chromatin shrinkage against nuclear envelope- PhykonosisCondensation of NucleusKaryorrhexis Degradation of DNA gap of NucleusBlebbing (Mathew et al).Localized decoupling of the cytoskeleton from plasma membranePhagocytosis or engulfing of dead cellsUsually present on the cytosolic surface but spread by scramblase carry over 1 Tabulated format of the observations during Apoptosis of the cell and their primary causes.2.5 Role of repressive or Promoter genes in ApoptosisThe cells after undergoing apoptosis in all tissues and animals appear similar and this cell death gets involved in many operations that are active and intracellular which can be promoted or inhibited by physiological or pathological stimuli.Regulation in Caenorhabditis elegansThe genes responsible for apoptosis was first identified in a nematode, Caenorhabditis elegans, related to cell death and its control (Horvitz et al., 1982 Ellis and Horvitz, 1986).Initial genetic studies in C.elegans led to the identification of a gene called Ced-3, a promoter gene responsible for programmed cell death to occur during the development of the worm (Ellis et al., 1991).The Ced-3 gene codes for an enzyme which is Cysteine Protease (Yuan et al., 1993).The gene cleaves the substrate after every active and specific aspartic acid sites and they get activated by cleaving at the aspartic acid sites. These are now referred to as Caspases (Alnemriet et al.,1996)The caspases mediate the apoptosis by cleaving at specific intracellular proteins that are of high selectivity and these proteins in turn activate the apoptosis process (Chinnaiyan and Dixit, 1996).Similarly there are many genes that inhibit the apoptosis process in the nematode, Caenorhabditis elegans. One such gene is Ced-9, which belongs to the same family as the Ced-3 and this gene inhibits apoptosis in the nematode (Hengartner and Horvitz, 1994).If Ced-9 is activated by open frame or any mutation, the worm dies at a very early stage when compared to the usual growth. Therefore Ced-9 is necessary to prevent programmed cell death if the cell has to survive in the developing worm. (Hengartner et al., 1992)Regulation in mammal cellsSimilar to the nematode there are many genes that act as inhibitors or promoters of programmed cell death or apoptosis in mammal cells also. They in turn contribute to organ and tissue development.Certain genes like Bcl- 2 and Bcl- XL act as inhibitors that inhibit programmed cell death.Genes like Bax and Bak act as promoter genes. They promote programmed cell or apoptosis.The just ratio of the inhibitors to the promoters determines the capacity of a mammalian cell to undergo apoptosis (Korsmeyer, 1995).Bcl- XL, in three dimensional structures is noted to function as a pore forming protein in the intercellular membrane where the genes are actually present. (Muchmore et al, 1996)When the Bcl- 2 and Bcl- XL is disrupted in a mammal like mice, the animal tends to die either as an embryo itself of in the post natal stage due to excessive programmed cell deaths in particular organs.When Bax is disrupted, normal programmed cell death or apoptosis process itself fails to occur (Deckwerth et al, 1996).Although proteins are required for the apoptosis process, inhibitors of RNA or protein synthesis often inhibit apoptosis indicating that transcription and translation are required to activate the programmed cell death process.2.6 Importance of Programmed Cell Death (Apoptosis)In the mutant nematodes, where the apoptosis process is deficient, it is f ound to have a normal life span. But whereas apoptosis process deficient flies are found to die at an early stage. The vertebrates exhibit results similar to that of the flies. This difference is due to the repressing and the promoter genes in the different organisms and their relation to organ development and tissue homeostasis.2.7 Consequences of Defective PathwaysThe consequences caused (diseases) are only caused by the big apoptotic pathways. Where normal apoptosis does not take place. The only way by which the flow of apoptosis is disrupted is by deferring the signal. When the pathway is inhibited the growth of the cell continues and the cells live more that they are supposed to actually live and differentiation of these cells also transfer the fault to their progeny. Which most probably leads to crabmeat. The inhibitor or the suppressor as we call it here binds to caspase preferably 8, 9 or 10 here in this case and stops the cell death.AIDS This specific viral protein deact ivates the anti-apoptotic Bcl-2 and triggers the mitochondrial regulation pathway to progress the reaction at a higher pace. FAS mediated apoptosis is increased and the death rate also increases (Perez et al., 2008).Cancer This disease is a causative of inhibition of apoptosis. Where the X-linked inhibitor of apoptosis protein plays a vital role. When the cells do not die at the specified time a tumor is produced leading to cancer (Ott et al., 2006 John and Kerr, 2006).Having mentioned about the disadvantages of the defective apoptotic pathways, the advantages of apoptosis is none other than the development of organs by programmed suicide of the cells As I have mentioned before, there are uncountable processes that are taking place inside an organism every milli second. The process of apoptosis has its own meaning amidst all the other. It can also be rightly named the mother of all processes as apoptosis is the cause of organ development and also the root cause of development of any organism.3. Organ DevelopmentOrgan development is also known as organogenesis. For our clear understanding we can explain it as the budding of organs from the growth cycle beginning from the form of a zygote. This routine is followed in all organisms which is just the consequence of death of cells in a programmable manner. The cells from the stage of division are never pre-determined the cells later are differentiated to form certain organs or organ systems. This programmed process of cell death coupled with organ development is the most important course of action in any organism as it determines the growth or death of the same. Then which can be followed by all the regular functions of the gene. To give a brief description about how organ development takes place, it is the proceedings of the ectoderm, endoderm and mesoderm to develop organs and organ systems. The embryo is at its weakest in this stage of development which may lead to anomalies or discretion.The soul reason behind this process on paper to progress in a timely manner is the proper differentiation of cells by gene coding. Which can literally imprint an impact on the cells to develop only to certain organs by coding them. Stem cells play the most vital part. Being the cells that can undergo easy differentiation combined with a superiorly faster rate of proliferation when compared to commonly proliferating cells.Figure 9 Development of Endoderm, Mesoderm and Ectoderm into specified organs by genetic coding. The layers are distinctively separated and differentiated into specific organs and organ systems by genetic regulation. This differentiation is defined by the genes that regulate the development.Endoderm Forms the tissue within the lungs and pancreas (Anne and Douglas, 2000).Mesoderm Function of forming muscles and also the tissues of kidneys.Ectoderm Primordial function of formation of tissues with the epidermis and most important characteristic of formation of neurons.3.1 Causes of unl awful organ developmentThere are many conditions that can cause improper organ development. Some of them like Toxicity, high amounts of radiation in the form of zygote or even in the higher order in the development line can cause permanent shift from the frame of reference. Other prime movers for this pursue are some like tobacco and alcohol and other headspring stimulating drugs. These obstructions of organ development due to mutation can been experiment on Arabidopsis thaliana and proved to cause the same effect on humans (Stein et al., 2004). This is because human field trials on this has been stated illegal all around the globe. Irregular apoptotic pathways can also be mentioned as some the reasons for the cause of improper organ development. But the root cause of everythig always lies in its beginning that is the primary infection of exposure to hazrdous materials or drugs. This can interfere in the primary pathway, leading inturn to the defective pathway of apoptosis. The de fective pathways in apoptosis can be the major cause of the second or improper organ development. May be considered as one of the most important reason for improper organ development.One of the most important reasons for improper organ developent is the cause of genetic mutations. The genetic mutations are caused by the radiotions or hazardous toxins that I have mentioned before. But what leads to improper organ development is genetic modification or mutations in the gene. This can lead to permanent damage of the cell or the gene. This gene damaged, specifies to an organ. ultimately the organ is completely damaged due to the mutation of the gene that tends to regulate that specific organ of the organism. Also considering the fact that the cells can also die due to mutations causing permanent damage in the organ development phase of the organism.4 Applications4.1 Clinical applicationsMany diseases like cancer, auto tolerant diseases, neuro-degenerative diseases do not either inhib it apoptosis or leads to contrary activation of apoptosis. They do not completely eliminate harmful cells which lead to loss of all the essential cells that prevent the oncoming of these diseases. Therefore, potential therapeutic strategies must be incorporated by including small molecules that either inhibit or activate certain target proteins that are responsible for apoptosis (Murphy et al., 2003).Generally there occurs a natural delay in the activation of Caspases after any injury and this delay allows enough time for treating the molecules that target Caspases. They are said to show therapeutic applications in preclinical studies (Reed, 2000 Nicholson, 2000). Bcl-2, another inhibitor gene of apoptosis, plays a vital role in the mitochondrial pathway and is regulated in many cancer cells. Introduction of an antisense Bcl-2 oligo molecule has shown promising results in preclinical trials in SCID mice and phase III clinical trials (Reed, 2000 Nicholson, 2000). There is something called, Inhibitors of Apoptosis (IAPs) that are of potential therapeutic targets for treatment of diseases. Some cancers over expresses the IAPs that is associated with the genes responsible for the resistance of apoptosis. One such gene is called Survivin (an IAP) which is involved in cancer cells. By eliminating this Survivin, the cancer cells become more sensitive to drugs that initiate apoptosis (Nicholson, 2000).4.2 Immunoblotting techniquesCytochrome C, an indicator of apoptosis is attached to the apoptotic cells along with the presence of genes responsible for apoptosis and immunoprecipitates were formed. By addition of anti-Cytochrome C or anti-Bc